Wahl M, Hermodsson S, Kjellman H, Iwarson S
Dev Biol Stand. 1983;54:341-5.
Hepatitis B Immunoglobulin with an antibody content of 2 300 IU was injected i.m. into five healthy female volunteers without Hepatitis B markers in serum (HBsAg, anti-HBs, anti-HBc). The uptake of anti-HBs after i.m. injection was calculated by measuring the concentration of antibody in plasma. An international standard (WHO) containing 100 IU/ml was used as reference. The serum concentration of anti-HBs was analysed on day 1, 3, 5, 7, 9, 11, 13, 28 and 56 after the single i.m. injection. The peak serum concentrations after one single injection were observed between day 5 and 11. The mean recovery rate was calculated according to a compartment model where the plasma volume was estimated to be 45 ml/kg body weight. The mean recovery rate expressed as percentage of injected dose was 19.2% with a range of 14.2-28.2%. The mean biological half life of anti-HBs was 21.7 days. The recovery rate of Hepatitis B Immunoglobulin injected i.m. seems to be somewhat lower than expected but is within the same range as i.e. Ig anti-D (Eklund et al, BMJ 1982, 284: 854-855). The peak serum concentration of anti-HBs was reached comparatively late after the injection. This finding may in part explain the discouraging protection of Hepatitis B Immunoglobulin in the post-exposure situation. Intravenous administration of anti-HBs with an immediate peak concentration followed by vaccination will possibly be a better alternative for post-exposure prophylaxis against Hepatitis B.
将抗体含量为2300国际单位的乙肝免疫球蛋白肌肉注射到5名血清中无乙肝标志物（乙肝表面抗原、乙肝表面抗体、乙肝核心抗体）的健康女性志愿者体内。通过测量血浆中抗体浓度来计算肌肉注射后乙肝表面抗体的摄取量。使用含100国际单位/毫升的国际标准品（世界卫生组织）作为参照。在单次肌肉注射后的第1、3、5、7、9、11、13、28和56天分析乙肝表面抗体的血清浓度。单次注射后的血清峰值浓度在第5天至第11天之间观察到。根据房室模型计算平均回收率，其中血浆体积估计为45毫升/千克体重。以注射剂量的百分比表示的平均回收率为19.2%，范围为14.2%至28.2%。乙肝表面抗体的平均生物学半衰期为21.7天。肌肉注射的乙肝免疫球蛋白的回收率似乎略低于预期，但与抗D免疫球蛋白（埃克隆德等人，《英国医学杂志》1982年，284:854 - 855）处于同一范围。注射后乙肝表面抗体的血清峰值浓度达到时间相对较晚。这一发现可能部分解释了乙肝免疫球蛋白在暴露后情况下令人沮丧的保护效果。静脉注射乙肝表面抗体并立即达到峰值浓度，随后进行疫苗接种可能是暴露后预防乙肝的更好选择。
