Sharma A K, Thomas P K, Gabriel G, Stolinski C, Dockery P, Hollins G W
Diabetes. 1983 Dec;32(12):1152-61. doi: 10.2337/diab.32.12.1152.
Observations were made on the genetically diabetic C57BL/Ks (db/db) mouse. Morphometric observations were performed on the tibial nerve at 6, 9, and 15 mo and compared with those from nondiabetic (m/m) controls. Myelinated fiber size was less in the diabetic animals at all stages and affected cross-sectional axon area and myelin thickness equally. Unmyelinated axons were unaffected. The index of circularity of myelinated axons did not differ between diabetic and control animals. No definite absolute reduction in fiber size occurred and degenerative changes, which were slight, were equally frequent in the diabetic and control nerves. Axonal glycogenosomes, polyglucosan particles, and Schwann cell/axon networks, and Schwann cell Reich granules increased with age in both groups, but only glycogenosomes were consistently more numerous in the diabetic animals. Counts of membrane associated particles in both P and E faces revealed that these were reduced in number in the diabetic animals in myelin and in axolemma of unmyelinated axons, but not in the axolemma of myelinated fibers. Growth in tibial length was also reduced in the diabetic animals and this suggested that the reduced fiber diameter probably represented a maturational deficit. The absence of a selective reduction in axonal size did not favor a primary axonopathy as the cause.
对遗传性糖尿病C57BL/Ks（db/db）小鼠进行了观察。在6个月、9个月和15个月时对胫神经进行形态测量观察，并与非糖尿病（m/m）对照小鼠的观察结果进行比较。在所有阶段，糖尿病动物的有髓纤维尺寸均较小，且对轴突横截面积和髓鞘厚度的影响相同。无髓轴突未受影响。糖尿病动物和对照动物之间有髓轴突的圆度指数没有差异。纤维尺寸没有明确的绝对减小，且两组神经中轻微的退行性变化出现频率相同。两组中轴突糖原小体、聚葡萄糖颗粒、雪旺细胞/轴突网络以及雪旺细胞 Reich 颗粒均随年龄增加，但只有糖原小体在糖尿病动物中始终更多。对P面和E面的膜相关颗粒计数显示，糖尿病动物有髓鞘和无髓轴突轴膜中的这些颗粒数量减少，但有髓纤维轴膜中没有减少。糖尿病动物的胫骨长度增长也减少，这表明纤维直径减小可能代表成熟缺陷。轴突大小没有选择性减小不支持原发性轴突病作为病因。
