Masihi K N, Brehmer W, Lange W, Ribi E
Int J Immunopharmacol. 1983;5(5):403-10. doi: 10.1016/0192-0561(83)90015-2.
The nonspecific protective effect in mice of pre-exposure to mycobacterial components and muramyl dipeptide three weeks before aerosol infection with influenza virus A/PR/8/34 (H1N1) was studied. Muramyl dipeptide, when combined with trehalose dimycolate and emulsified in an oil-in-water emulsion, conferred complete protection comparable to specific immunization with a high dose of formalin inactivated A/PR/8/34 influenza viral vaccine. Animals pre-exposed to muramyl dipeptide plus trehalose dimycolate showed a marked reduction in lung virus titres, an earlier clearance of detectable infectious virus, and an earlier onset of antibody production in comparison to control mice. Resistance to infection was also observed with BCG-cell wall skeleton combined with trehalose dimycolate and trehalose dimycolate alone when given as oil-in-water preparations. The route of administration of nonspecific stimulants was crucial. Only intravenous but not intradermal inoculation produced significant protection.
研究了在小鼠经气溶胶感染甲型流感病毒A/PR/8/34（H1N1）三周前预先接触分枝杆菌成分和胞壁酰二肽的非特异性保护作用。胞壁酰二肽与海藻糖二霉菌酸酯结合并乳化于水包油乳剂中时，可提供与高剂量甲醛灭活的A/PR/8/34流感病毒疫苗特异性免疫相当的完全保护。与对照小鼠相比，预先接触胞壁酰二肽加海藻糖二霉菌酸酯的动物肺病毒滴度显著降低，可检测到的传染性病毒清除更早，抗体产生也更早。当以水包油制剂形式给予时，卡介苗细胞壁骨架与海藻糖二霉菌酸酯以及单独的海藻糖二霉菌酸酯也观察到了对感染的抵抗力。非特异性刺激剂的给药途径至关重要。只有静脉注射而非皮内接种能产生显著的保护作用。
