Masihi K N, Lange W, Rohde-Schulz B
Cancer Immunol Immunother. 1987;24(1):19-24. doi: 10.1007/BF00199828.
The effect on natural killer (NK) cytotoxicity of splenic cells from BALB/c mice pretreated i.v. with squalane-in-water preparations of muramyl dipeptide (MDP), trehalose dimycolate (TDM), or the combination of MDP-plus-TDM was investigated. MDP or TDM augmented the NK cytotoxicity which peaked 48 h after the pretreatment whereas the combination of MDP and TDM induced an inhibition of the NK activity. Infection with influenza virus, a potent stimulator of NK cells, after the pretreatment with biological response modifiers resulted in a markedly enhanced NK activity on day 2 in MDP and control groups. Mice pretreated with TDM or the combination of MDP and TDM showed only moderate NK activity which peaked on day 3 after influenza infection. The NK activity was susceptible to asialo GM1 and complement treatment. The cytotoxicity of MDP-plus-TDM cells could be significantly enhanced after treatment with anti-macrophage monoclonal antibody and complement. NK activity induced by MDP or TDM was reduced by mixing MDP-plus-TDM cells. Addition of adherent cell-depleted MDP-plus-TDM suspension to MDP or TDM cells had a NK restorative effect. Splenic cells from mice pretreated 2 days earlier with MDP or TDM, but not MDP-plus-TDM, generated enhanced levels of luminol-dependent chemiluminescence.
研究了静脉注射用的水包角鲨烯制剂的胞壁酰二肽（MDP）、海藻糖二霉菌酸酯（TDM）或MDP加TDM组合预处理的BALB/c小鼠脾细胞对自然杀伤（NK）细胞毒性的影响。MDP或TDM增强了NK细胞毒性，在预处理后48小时达到峰值，而MDP和TDM的组合则诱导了NK活性的抑制。在用生物反应调节剂预处理后，感染流感病毒（一种NK细胞的有效刺激剂），在MDP组和对照组中，第2天NK活性显著增强。用TDM或MDP与TDM组合预处理的小鼠仅表现出中等程度的NK活性，在流感感染后第3天达到峰值。NK活性对去唾液酸GM1和补体处理敏感。用抗巨噬细胞单克隆抗体和补体处理后，MDP加TDM细胞的细胞毒性可显著增强。通过混合MDP加TDM细胞，MDP或TDM诱导的NK活性降低。将去除贴壁细胞的MDP加TDM悬浮液添加到MDP或TDM细胞中具有NK恢复作用。提前2天用MDP或TDM预处理的小鼠的脾细胞，但不是MDP加TDM预处理的小鼠，产生了增强水平的鲁米诺依赖性化学发光。
