Masihi K N, Brehmer W, Azuma I, Lange W, Müller S
Infect Immun. 1984 Jan;43(1):233-7. doi: 10.1128/iai.43.1.233-237.1984.
The effect on respiratory burst of splenic cells from mice pretreated with oil-in-water emulsions of muramyl dipeptide (MDP), trehalose dimycolate (TDM), or the combination of MDP with TDM was studied by luminol-dependent chemiluminescence in response to stimulation by zymosan. Spleen cells from mice pretreated with TDM, but not those of mice treated with MDP, generated increased chemiluminescence. Spleen cells from animals pretreated with the combination of MDP and TDM exhibited markedly enhanced chemiluminescence activity. The effect of enhanced activity of preparations containing MDP combined with TDM was further examined in vivo by an aerosol infection of pretreated mice with a mouse-pathogenic influenza virus. Pretreatment with 6-O-acyl analogs and one ubiquinone derivative of MDP alone did not induce any resistance against influenza virus. Significant protection was conferred only when MDP and certain analogs were combined with TDM. The enhancement of nonspecific resistance to influenza virus infection was related to the chemical structure of the synthetic immunostimulant. A greater degree of protection was induced by the combination of TDM with the lipophilic derivatives like B 30-MDP and L-18 MDP.
通过鲁米诺依赖性化学发光法，研究了用胞壁酰二肽（MDP）、海藻糖二霉菌酸酯（TDM）或MDP与TDM的组合的水包油乳液预处理的小鼠脾细胞对酵母聚糖刺激的呼吸爆发的影响。用TDM预处理的小鼠的脾细胞产生了增强的化学发光，而用MDP处理的小鼠的脾细胞则没有。用MDP和TDM组合预处理的动物的脾细胞表现出明显增强的化学发光活性。通过用小鼠致病性流感病毒气溶胶感染预处理的小鼠，在体内进一步研究了含有MDP与TDM组合制剂的增强活性的效果。单独用MDP的6-O-酰基类似物和一种泛醌衍生物预处理不会诱导对流感病毒的任何抗性。只有当MDP和某些类似物与TDM组合时才会产生显著的保护作用。对流感病毒感染的非特异性抗性增强与合成免疫刺激剂的化学结构有关。TDM与亲脂性衍生物如B 30-MDP和L-18 MDP的组合诱导了更大程度的保护作用。
