使用液体闪烁计数器通过洛瑞技术进行蛋白质的比色测定。
The colorimetric estimation of protein by the Lowry technique using a liquid scintillation counter.
作者信息
Noble R C, Shand J H
出版信息
J Biochem Biophys Methods. 1983 Nov;8(3):223-6. doi: 10.1016/0165-022x(83)90070-2.
A comparison has been made of the use of the spectrophotometer and liquid scintillation counter for the colorimetric quantification of protein. The attenuation of photon detection from sealed miniature 14C radioactive standards enabled protein quantification over a concentration range far in excess of that achievable by the use of a spectrophotometer. Accuracy of quantification was high over the entire range of protein concentration. The ability of the technique to provide quick and accurate protein estimations is discussed.
已对使用分光光度计和液体闪烁计数器进行蛋白质比色定量分析进行了比较。密封微型14C放射性标准品的光子检测衰减使得能够在远超分光光度计所能达到的浓度范围内进行蛋白质定量。在整个蛋白质浓度范围内，定量的准确性都很高。本文讨论了该技术提供快速准确蛋白质估计的能力。
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