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1
Structure and expression of the Chinese hamster thymidine kinase gene.中国仓鼠胸苷激酶基因的结构与表达
Mol Cell Biol. 1986 Jun;6(6):1998-2010. doi: 10.1128/mcb.6.6.1998-2010.1986.
2
Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5反应性表达的遗传决定因素包含在胸苷激酶mRNA序列中。
Mol Cell Biol. 1986 Jun;6(6):2262-6. doi: 10.1128/mcb.6.6.2262-2266.1986.
3
Isolation and preliminary characterization of the Chinese hamster thymidine kinase gene.中国仓鼠胸苷激酶基因的分离与初步鉴定。
Mol Cell Biol. 1983 Oct;3(10):1815-23. doi: 10.1128/mcb.3.10.1815-1823.1983.
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Molecular cloning and structural analysis of murine thymidine kinase genomic and cDNA sequences.小鼠胸苷激酶基因组和cDNA序列的分子克隆及结构分析
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Characterization of the murine thymidine kinase-encoding gene and analysis of transcription start point heterogeneity.小鼠胸苷激酶编码基因的特征分析及转录起始点异质性分析。
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Transcriptional and posttranscriptional mechanisms regulate murine thymidine kinase gene expression in serum-stimulated cells.转录和转录后机制调节血清刺激细胞中鼠胸苷激酶基因的表达。
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A processed gene homologous to the thymidine kinase (TK) gene in Chinese hamster cells.一种与中国仓鼠细胞中的胸苷激酶（TK）基因同源的加工基因。
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Genomic organization and linkage via a bidirectional promoter of the AP-3 (adaptor protein-3) mu3A and AK (adenosine kinase) genes: deletion mutants of AK in Chinese hamster cells extend into the AP-3 mu3A gene.AP-3（衔接蛋白-3）μ3A基因与AK（腺苷激酶）基因通过双向启动子的基因组组织与连锁关系：中国仓鼠细胞中AK的缺失突变体延伸至AP-3 μ3A基因。
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Transfer of genes to Chinese hamster ovary cells by DNA-mediated transformation.通过DNA介导的转化将基因转移到中国仓鼠卵巢细胞中。
Somatic Cell Genet. 1982 Jan;8(1):23-39. doi: 10.1007/BF01538648.

引用本文的文献

1
Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA.稳定的小鼠胸苷激酶mRNA的细胞周期调控合成是由cDNA中的一个序列介导的。
Nucleic Acids Res. 1987 Jan 26;15(2):741-52. doi: 10.1093/nar/15.2.741.
2
Control of thymidine kinase mRNA during the cell cycle.细胞周期中胸苷激酶信使核糖核酸的调控
Mol Cell Biol. 1987 Aug;7(8):2925-32. doi: 10.1128/mcb.7.8.2925-2932.1987.
3
Cell-cycle-specific interaction of nuclear DNA-binding proteins with a CCAAT sequence from the human thymidine kinase gene.核DNA结合蛋白与人胸苷激酶基因CCAAT序列的细胞周期特异性相互作用。
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8350-4. doi: 10.1073/pnas.84.23.8350.
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Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.内含子对小鼠L细胞中细胞胸苷激酶mRNA的有效形成无关紧要。
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Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.人类胸苷激酶基因启动子中包含的序列可指导异源融合基因的细胞周期调控。
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Do exons code for structural or functional units in proteins?外显子是否编码蛋白质中的结构或功能单元？
Proc Natl Acad Sci U S A. 1988 May;85(9):2944-8. doi: 10.1073/pnas.85.9.2944.
7
Transcriptional and posttranscriptional mechanisms regulate murine thymidine kinase gene expression in serum-stimulated cells.转录和转录后机制调节血清刺激细胞中鼠胸苷激酶基因的表达。
Mol Cell Biol. 1988 Dec;8(12):5280-91. doi: 10.1128/mcb.8.12.5280-5291.1988.
8
Genetic determinants of growth phase-dependent and adenovirus 5-responsive expression of the Chinese hamster thymidine kinase gene are contained within thymidine kinase mRNA sequences.中国仓鼠胸苷激酶基因的生长阶段依赖性和腺病毒5反应性表达的遗传决定因素包含在胸苷激酶mRNA序列中。
Mol Cell Biol. 1986 Jun;6(6):2262-6. doi: 10.1128/mcb.6.6.2262-2266.1986.
9
Identification and nucleotide sequence of the thymidine kinase gene of Shope fibroma virus.肖普纤维瘤病毒胸苷激酶基因的鉴定及核苷酸序列
J Virol. 1986 Dec;60(3):920-7. doi: 10.1128/JVI.60.3.920-927.1986.
10
Mouse thymidine kinase: the promoter sequence and the gene and pseudogene structures in normal cells and in thymidine kinase deficient mutants.小鼠胸苷激酶：正常细胞以及胸苷激酶缺陷型突变体中的启动子序列、基因和假基因结构
Nucleic Acids Res. 1989 Jan 11;17(1):185-95. doi: 10.1093/nar/17.1.185.

本文引用的文献

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Temporal coordination of DNA replication with enzyme synthesis in diploid and heteroploid cells.二倍体细胞和异倍体细胞中DNA复制与酶合成的时间协调。
Science. 1969 Dec 19;166(3912):1536-8. doi: 10.1126/science.166.3912.1536.
2
ACTIVITIES OF THYMIDINE KINASE AND THYMINE DEOXYRIBONUCLEOTIDE PHOSPHATASE DURING GROWTH OF CELLS IN TISSUE CULTURE.组织培养中细胞生长期间胸苷激酶和胸腺嘧啶脱氧核苷酸磷酸酶的活性
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3
DECLINE OF THYMIDINE KINASE ACTIVITY IN STATIONARY PHASE MOUSE FIBROBLAST CELLS.静止期小鼠成纤维细胞中胸苷激酶活性的下降
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STUDIES ON THYMIDINE KINASE IN CULTURED MOUSE FIBROBLASTS.培养的小鼠成纤维细胞中胸苷激酶的研究。
Biochim Biophys Acta. 1965 Jan 11;95:14-22. doi: 10.1016/0005-2787(65)90206-6.
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Ovomucoid intervening sequences specify functional domains and generate protein polymorphism.卵类黏蛋白间隔序列决定功能域并产生蛋白质多态性。
Cell. 1980 Oct;21(3):681-7. doi: 10.1016/0092-8674(80)90431-6.
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Asymmetrical distribution of CpG in an 'average' mammalian gene.“普通”哺乳动物基因中CpG的不对称分布。
Nucleic Acids Res. 1982 Dec 11;10(23):7865-77. doi: 10.1093/nar/10.23.7865.
7
Regulation of thymidine kinase enzyme level in serum-stimulated mouse 3T6 fibroblasts.血清刺激的小鼠3T6成纤维细胞中胸苷激酶酶水平的调节。
Exp Cell Res. 1982 Mar;138(1):79-85. doi: 10.1016/0014-4827(82)90093-3.
8
A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
9
Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.缩写的小鼠二氢叶酸还原酶基因在培养的仓鼠细胞中的表达。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6522-6. doi: 10.1073/pnas.79.21.6522.
10
Cell cycle-dependent regulation of thymidine kinase activity introduced into mouse LMTK- cells by DNA and chromatin-mediated gene transfer.通过DNA和染色质介导的基因转移导入小鼠LMTK-细胞的胸苷激酶活性的细胞周期依赖性调控。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1119-23. doi: 10.1073/pnas.78.2.1119.

中国仓鼠胸苷激酶基因的结构与表达

Structure and expression of the Chinese hamster thymidine kinase gene.

作者信息

Lewis J A

出版信息

Mol Cell Biol. 1986 Jun;6(6):1998-2010. doi: 10.1128/mcb.6.6.1998-2010.1986.

DOI:10.1128/mcb.6.6.1998-2010.1986
PMID:3453109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367739/
Abstract

My colleagues and I have cloned a nearly full-length Chinese hamster thymidine kinase (TK) cDNA in a lambda gt10 vector and characterized this cDNA by nucleotide sequencing. The hamster TK protein is encoded in this cDNA by a 702-base-pair open reading frame which specifies a 25,625-dalton protein closely homologous to the previously described human and chicken TK proteins. Using cDNA nucleotide sequence data in conjunction with sequence data derived from selected subclones of the hamster TK gene recombinant phage lambda HaTK.5, we have resolved the structure of the TK gene, finding the 1,219 base pairs of the cDNA sequence to be distributed through 11.2 kilobases of genomic DNA in at least seven exon segments. In addition, we have constructed a variety of Chinese hamster TK minigenes and exonuclease III-S1 derivatives of these genes which have permitted us to define the limits of the Chinese hamster TK gene promoter and demonstrate that efficient TK transformation of Ltk- cells by TK minigenes depends on the presence of both TK intervening sequences and sequences 3' to the site of mRNA polyadenylation.

摘要

我和我的同事在λgt10载体中克隆了一个近乎全长的中国仓鼠胸苷激酶（TK）cDNA，并通过核苷酸测序对该cDNA进行了表征。仓鼠TK蛋白由这个cDNA中的一个702个碱基对的开放阅读框编码，该开放阅读框指定了一个25,625道尔顿的蛋白，与先前描述的人类和鸡的TK蛋白密切同源。利用cDNA核苷酸序列数据以及来自仓鼠TK基因重组噬菌体λHaTK.5的选定亚克隆的序列数据，我们解析了TK基因的结构，发现cDNA序列的1219个碱基对分布在至少7个外显子片段中的11.2千碱基的基因组DNA中。此外，我们构建了多种中国仓鼠TK微型基因以及这些基因的核酸外切酶III-S1衍生物，这使我们能够确定中国仓鼠TK基因启动子的界限，并证明TK微型基因对Ltk-细胞的有效TK转化取决于TK间隔序列和mRNA聚腺苷酸化位点3'端序列的存在。