Nathaniel D, Mellors A
Mol Immunol. 1983 Dec;20(12):1259-65. doi: 10.1016/0161-5890(83)90154-2.
Mitogenic and non-mitogenic lectins have been compared for their abilities to stimulate acetate and choline incorporation into mouse spleen lymphocyte lipids. The concns required for maximal acetate incorporation correspond to those required for maximal blastogenesis. The non-mitogenic lectins tested had no effect on acetate incorporation. Concanavalin A also stimulated acetate incorporation into splenic lymphocyte lipids of athymic mice which do not undergo T-cell blastogenesis. 4,4'-Diaminodiphenylsulfone (dapsone) at 150 micrograms/ml inhibited the incorporation of choline into mouse spleen lymphocyte phosphatidylcholine by 40%, but had no significant effect on concanavalin A induced mitogenesis when the inhibitor was present during the first few hours of transformation. Enhanced turnover of phospholipids appears to be a parallel but non-essential event in the early stages of mitogenesis.
已对促有丝分裂和非促有丝分裂凝集素刺激醋酸盐和胆碱掺入小鼠脾脏淋巴细胞脂质的能力进行了比较。最大程度掺入醋酸盐所需的浓度与最大程度细胞分裂所需的浓度相对应。所测试的非促有丝分裂凝集素对醋酸盐掺入没有影响。伴刀豆球蛋白A也刺激醋酸盐掺入无胸腺小鼠脾脏淋巴细胞脂质中，这些小鼠不会发生T细胞分裂。150微克/毫升的4,4'-二氨基二苯砜（氨苯砜）可使胆碱掺入小鼠脾脏淋巴细胞磷脂酰胆碱的量减少40%，但在转化的最初几个小时存在抑制剂时，对伴刀豆球蛋白A诱导的有丝分裂没有显著影响。磷脂周转增强似乎是有丝分裂早期的一个平行但非必需的事件。
