Koizumi S, Sone T, Kimura M, Otsuka F, Ohsawa M
Department of Experimental Toxicology, National Institute of Industrial Health, Kawasaki, Japan.
Experientia Suppl. 1987;52:519-23. doi: 10.1007/978-3-0348-6784-9_52.
Monocytes and lymphocytes were separated from human peripheral blood, and their capacity to synthesize metallothioneins (MTs) was examined. Both cell types can produce proteins electrophoretically similar to HeLa cell MTs. These proteins are inducible by Cd2+ and specifically precipitable by anti-MT serum. To have an insight into the capacity of T- and B-lymphocytes to produce MTs, sensitivity to Cd2+ of both cell types was estimated during mitogen-specific blastogenesis. Lymphocytes were prepared from mouse spleen and stimulated by concanavalin A (ConA) or lipopolysaccharide (LPS) in the presence of Cd2+. [3H]thymidine incorporation, as well as [3H]uridine incorporation in an earlier stage, was more severely inhibited by Cd2+ in ConA-induced mitogenesis than in LPS-induced mitogenesis, indicating T-lymphocytes are more sensitive to Cd2+ toxicity. This may reflect lower capacity of T-lymphocytes to synthesize MTs in response to Cd2+.
从人外周血中分离出单核细胞和淋巴细胞，并检测它们合成金属硫蛋白（MTs）的能力。这两种细胞类型都能产生在电泳上与HeLa细胞MTs相似的蛋白质。这些蛋白质可被Cd2+诱导，并能被抗MT血清特异性沉淀。为了深入了解T淋巴细胞和B淋巴细胞产生MTs的能力，在有丝分裂原特异性细胞增殖过程中评估了这两种细胞类型对Cd2+的敏感性。从小鼠脾脏制备淋巴细胞，并在Cd2+存在的情况下用刀豆球蛋白A（ConA）或脂多糖（LPS）刺激。在ConA诱导的有丝分裂过程中，[3H]胸腺嘧啶核苷掺入以及早期的[3H]尿嘧啶核苷掺入受到Cd2+的抑制比在LPS诱导的有丝分裂过程中更严重，这表明T淋巴细胞对Cd2+毒性更敏感。这可能反映出T淋巴细胞响应Cd2+合成MTs的能力较低。
