Kunisada T, Yamagishi H, Sekiguchi T
Plasmid. 1983 Nov;10(3):242-50. doi: 10.1016/0147-619x(83)90038-0.
For determination of the cellular location of small polydisperse circular DNA complexes, rat myoblastic L6 cells, HeLa cells, and mouse L cells were enucleated and processed by the micapress-adsorption method for electron microscopy (H. Yamagishi, T. Kunisada, and T. Tsuda, 1982, Plasmid 8, 299-306). Small circular DNA complexes from intact cells showed a heterogeneous size distribution of from 0.1 to more than 2 micron with a mean contour length of 0.6 to 0.8 micron, like that of covalently closed circular DNAs. Cells contained 400 to 1200 copies. The size distribution in the cytoplasts was narrow and the number-average length was 0.3 to 0.4 micron, whereas that in L6 karyoplasts was wide and the average length was 0.9 micron. The longer circular complexes appeared to be absent from the cytoplasts. The origin and biological functions of these complexes are discussed in relation to the cellular locations of the complexes.
为了确定小的多分散环状DNA复合物的细胞定位，对大鼠成肌L6细胞、HeLa细胞和小鼠L细胞进行去核处理，并采用微压吸附法进行电子显微镜观察（H. Yamagishi、T. Kunisada和T. Tsuda，1982年，《质粒》8，299 - 306）。完整细胞中的小环状DNA复合物呈现出0.1至2微米以上的异质大小分布，平均轮廓长度为0.6至0.8微米，与共价闭合环状DNA相似。细胞含有400至1200个拷贝。胞质体中的大小分布较窄，数均长度为0.3至0.4微米，而L6核质体中的大小分布较宽，平均长度为0.9微米。胞质体中似乎不存在较长的环状复合物。结合这些复合物的细胞定位，对这些复合物的起源和生物学功能进行了讨论。
