来自小鼠胸腺细胞的染色体外环状DNA中的类转座子序列。
Transposon-like sequences in extrachromosomal circular DNA from mouse thymocytes.
作者信息
Fujimoto S, Tsuda T, Toda M, Yamagishi H
出版信息
Proc Natl Acad Sci U S A. 1985 Apr;82(7):2072-6. doi: 10.1073/pnas.82.7.2072.
Abstract
Small polydisperse circular (spc) DNA was isolated from mouse thymocytes and cloned into the HindIII site of lambda vector Charon 7. Fifty-six recombinants from this spc DNA library were analyzed. R repeats, which were originally found near immunoglobulin genes, were enriched in spc DNA clones relative to their representation in the chromosome. In one clone, the R sequence was linked to Bam and MIF sequences and the contiguous arrangement was truncated from both ends. In another clone, composite Bam/R and R repeats existed as a pair in inverted repeat orientation. Truncation occurred from the 5' side without affecting the 3' ends. In both clones, short direct repeats flanked the repeated sequences. The possible role of R sequences in transposition and circular formation is discussed.
摘要
从小鼠胸腺细胞中分离出小的多分散环状（spc）DNA，并将其克隆到λ载体Charon 7的HindIII位点。对该spc DNA文库中的56个重组体进行了分析。最初在免疫球蛋白基因附近发现的R重复序列，相对于其在染色体中的表现，在spc DNA克隆中富集。在一个克隆中，R序列与Bam和MIF序列相连，并且连续排列从两端被截断。在另一个克隆中，复合Bam/R和R重复序列以反向重复方向成对存在。截断发生在5'端，而不影响3'端。讨论了R序列在转座和环状形成中的可能作用。
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