来自小鼠造血组织细胞的染色体外环状DNA。

Extrachromosomal circular DNAs from murine hemopoietic tissue cells.

作者信息

Tsuda T, Yamagishi H, Ohnishi N, Yamada Y, Izumi H, Mori K J

出版信息

Plasmid. 1983 Nov;10(3):235-41. doi: 10.1016/0147-619x(83)90037-9.

Abstract

Extrachromosomal circular DNA complexes from cells of murine hemopoietic organs, bone marrow, thymus, spleen, and lymph nodes were examined by mica-press-adsorption method (H. Yamagishi, T. Kunisada, and T. Tsuda, 1982, Plasmid 8, 299-306). They showed wide size distribution, from 0.3 to 10 micron. The large-size DNAs of more than 1 micron (3.1 kb) in contour length were more abundant in bone marrow and thymus than they were in spleen and lymph nodes. The appearance of the large size DNAs was examined on splenocytes of athymic nude mice during ontogeny. The large-size DNAs first became detectable after 2 weeks of age and the amount increased thereafter until 9 weeks of age. It appears that large-size circular DNAs appear during differentiation from the hemopoietic stem cells into several descendent cells. Possible immunological implications for the appearance of extrachromosomal circular DNAs are discussed.

摘要

采用云母压片吸附法（H. 山岸、T. 国定、T. 津田，1982年，《质粒》8卷，第299 - 306页）对来自小鼠造血器官、骨髓、胸腺、脾脏和淋巴结细胞的染色体外环状DNA复合物进行了检测。它们呈现出广泛的大小分布，从0.3微米到10微米不等。轮廓长度超过1微米（3.1 kb）的大尺寸DNA在骨髓和胸腺中比在脾脏和淋巴结中更为丰富。在无胸腺裸鼠的脾细胞发育过程中，对大尺寸DNA的出现情况进行了检测。大尺寸DNA在2周龄后首次可检测到，此后数量增加，直至9周龄。似乎大尺寸环状DNA在造血干细胞向几种后代细胞分化的过程中出现。文中讨论了染色体外环状DNA出现可能的免疫学意义。