Wahl R, Maasch H J, Geissler W
Anal Biochem. 1983 Oct 1;134(1):189-94. doi: 10.1016/0003-2697(83)90282-8.
Three variations of the radioallergosorbent (RAST)-inhibition assay (RI) have been compared for measuring the allergenic activity of grass pollen extracts. The main difference between the three assays consisted in the solid supports to which the allergens were coupled. These supports were paper disks, microcrystalline cellulose, and Matrex. It could be demonstrated that all three variations of RI yielded almost identical results as expressed by their F value (F0). Correlations between the three methods were highly significant (P less than 0.001). Three independent valid assays showed an excellent reproducibility of each test system (P less than 0.01). Three different preparations of each of the supports yielded highly reproducible F0 (0.76 +/- 3% SD). Evaluation of the 50% inhibition values achieved with the different assays based on protein showed differences between the three supports. Provided the same allergen extract is coupled to the different solid supports and the results are related to a reference preparation, different laboratories using different forms of RI will be able to compare their results.
已对放射变应原吸附试验（RAST）抑制测定法（RI）的三种变体进行了比较，以测量草花粉提取物的变应原活性。这三种测定法之间的主要区别在于与变应原偶联的固相支持物。这些支持物是纸盘、微晶纤维素和Matrex。可以证明，RI的所有三种变体产生的结果几乎相同，以其F值（F0）表示。三种方法之间的相关性非常显著（P小于0.001）。三个独立的有效测定法显示每个测试系统具有出色的重现性（P小于0.01）。每种支持物的三种不同制剂产生高度可重现的F0（0.76±3%标准差）。基于蛋白质的不同测定法所获得的50%抑制值的评估显示三种支持物之间存在差异。如果将相同的变应原提取物偶联到不同的固相支持物上，并且结果与参考制剂相关，则使用不同形式RI的不同实验室将能够比较他们的结果。
