Batenburg J J, Ossendorp B C, Snoek G T, Wirtz K W, Houweling M, Elfring R H
Laboratory of Veterinary Biochemistry, Utrecht University, The Netherlands.
Biochem J. 1994 Feb 15;298 ( Pt 1)(Pt 1):223-9. doi: 10.1042/bj2980223.
Gene expression of non-specific lipid-transfer protein (nsL-TP; identical with sterol carrier protein 2) and phosphatidylinositol-transfer protein (PI-TP) was investigated in developing rat lung. During the late prenatal period (between days 17 and 22) there is a 7-fold increase in the level of nsL-TP and a 2-fold rise in that of PI-TP. The prenatal increases in the levels of nsL-TP and PI-TP are accompanied by parallel increases in the levels of their mRNAs, indicating pretranslational regulation. Compared with whole lung, isolated alveolar type-II cells are enriched in nsL-TP and its mRNA, but not in PI-TP and its mRNA. The observation that the levels of nsL-TP and its mRNA in rat lung show a pronounced increase in the period of accelerated surfactant formation, together with the observation that the surfactant-producing type-II cells are enriched in nsL-TP and its mRNA, suggest that nsL-TP plays a role in the metabolism of pulmonary surfactant.
研究了发育中大鼠肺中非特异性脂质转运蛋白（nsL-TP；与固醇载体蛋白2相同）和磷脂酰肌醇转运蛋白（PI-TP）的基因表达。在产前后期（第17至22天之间），nsL-TP水平增加了7倍，PI-TP水平增加了2倍。产前nsL-TP和PI-TP水平的增加伴随着它们mRNA水平的平行增加，表明存在翻译前调控。与全肺相比，分离的II型肺泡细胞中nsL-TP及其mRNA含量丰富，但PI-TP及其mRNA含量不丰富。大鼠肺中nsL-TP及其mRNA水平在表面活性剂形成加速期显著增加，以及产生表面活性剂的II型细胞中nsL-TP及其mRNA含量丰富，这些观察结果表明nsL-TP在肺表面活性剂的代谢中起作用。
