Yamamoto T, Kihara I, Hara M, Kawasaki K, Yaoita E
Br J Exp Pathol. 1983 Dec;64(6):660-9.
We introduced a mesangiopathic form of glomerulonephritis in spontaneously hypertensive (SHR) rats. Bovine serum albumin (BSA) was given i.v. to primed rats for 3 weeks and they were unilaterally nephrectomized (Nx). Then, they received rabbit anti-BSA- (Group A) or normal serum (Group B) for seven days, and half the rats were killed to obtain another kidney (Ex-1). The remainder were killed two weeks later and their kidneys were examined (Ex-2). In Nx kidneys, the glomerular lesions were characterized by leucocyte accumulation in the capillary lumina and by deposition of rat IgG, rat C3 and BSA both in the mesangial area and along the capillary walls. Glomeruli of Ex-1 kidneys manifested varying degrees of hypercellularity in the mesangium; a few leucocyte accumulations in the capillary lumina were noted and the immune deposits had decreased in the mesangium but not on the capillary walls. In Ex-2 kidneys, mesangial hypercellularity was conspicuous. There were no remarkable histological differences between Group A and B rats; in Ex-1 and Ex-2 kidneys of Group A, rabbit IgG was closely associated with rat IgG or C3. Serological evaluation revealed that the amount of circulating rat anti-BSA antibody was relatively small and that C3 was consumed by newly formed circulating immune complexes during BSA administration. Polymorphonuclear leucocyte (PMN) binding assay revealed that complement fixation to the immune deposits occurred in vitro and that this activity was highest in tissue from Nx kidneys.
我们在自发性高血压（SHR）大鼠中诱发了一种系膜病性肾小球肾炎。给致敏大鼠静脉注射牛血清白蛋白（BSA），持续3周，然后对其进行单侧肾切除（Nx）。之后，它们接受兔抗BSA（A组）或正常血清（B组）注射，持续7天，然后处死一半大鼠以获取另一侧肾脏（Ex-1）。其余大鼠在两周后处死并检查其肾脏（Ex-2）。在Nx肾脏中，肾小球病变的特征是毛细血管腔内白细胞积聚，以及大鼠IgG、大鼠C3和BSA在系膜区和毛细血管壁沉积。Ex-1肾脏的肾小球系膜表现出不同程度的细胞增多；在毛细血管腔内观察到少量白细胞积聚，系膜区的免疫沉积物减少，但毛细血管壁上的免疫沉积物未减少。在Ex-2肾脏中，系膜细胞增多明显。A组和B组大鼠之间没有显著的组织学差异；在A组的Ex-1和Ex-2肾脏中，兔IgG与大鼠IgG或C3紧密相关。血清学评估显示，循环中的大鼠抗BSA抗体量相对较少，并且在注射BSA期间，新形成的循环免疫复合物消耗了C₃。多形核白细胞（PMN）结合试验显示，免疫沉积物在体外发生补体固定，并且这种活性在Nx肾脏组织中最高。
