Hattori M, Sakamoto K, Wakabayashi K
Endocrinol Jpn. 1983 Jun;30(3):289-96. doi: 10.1507/endocrj1954.30.289.
The isoelectric components of LH have been isolated from the male rat pituitary glands by preparative isoelectric focusing. The biological activities of these components were measured in vitro from testosterone production in rat Leydig cells at equal doses and expressed as the equivalent of NIH-LH-S1 immunoreactivity. There were significant differences in the bioactivities among the components. The bioactivities of the components decreased with decreasing pI; components E (pI = 9.6) and F (pI = 9.8) showed about 5- to 6-fold higher activities than component A' (pI = 7.9). When the rat pituitary extracts were measured for LH by radioimmunoassay, and then measured for the ratio of biological activity to immunoreactivity (B:I), the order was as follows; intact female rats greater than intact male rats greater than orchidectomized rats. This phenomenon could be explained by the differences in the relative amounts of the LH components which changed according to the physiological states. These observations indicate that the LH components with different pIs and B:I ratio are related to the different estimation of total LH in the rat pituitary glands by bioassay and radioimmunoassay.
通过制备性等电聚焦从雄性大鼠垂体中分离出促黄体激素（LH）的等电成分。这些成分的生物活性在体外以等剂量大鼠睾丸间质细胞中睾酮的产生量来测定，并表示为相当于NIH-LH-S1免疫反应性的量。各成分之间的生物活性存在显著差异。成分的生物活性随等电点（pI）降低而降低；成分E（pI = 9.6）和F（pI = 9.8）的活性比成分A'（pI = 7.9）高约5至6倍。当通过放射免疫测定法测量大鼠垂体提取物中的LH，然后测量生物活性与免疫反应性的比率（B:I）时，顺序如下：完整雌性大鼠＞完整雄性大鼠＞去势大鼠。这种现象可以通过根据生理状态而变化的LH成分相对含量的差异来解释。这些观察结果表明，具有不同pI和B:I比率的LH成分与通过生物测定法和放射免疫测定法对大鼠垂体中总LH的不同估计有关。
