Widdicombe J H, Coleman D L, Finkbeiner W E, Friend D S
Cell Tissue Res. 1987 Jan;247(1):95-103. doi: 10.1007/BF00216551.
Cells from the dog's tracheal mucosa formed confluent epithelial sheets in culture. Typical tight junctions separated the apical from the basolateral portion of the cell membrane. The apical portion of the cell contained numerous short microvilli and a pronounced glycocalyx. The basolateral portion of the plasma membrane was unspecialized except for extensive gap junctions between cells. Freeze-fracture showed that the cultured cells lacked the basolateral square (orthogonal) arrays of the original tissue, particles previously implicated in ion transport. Formation of domes indicated the presence of active fluid absorption. Domes appeared between days 4 and 8 of culture and persisted for about 1 week. Cell sheets showed a transepithelial resistance of approximately equal to 400 omega X cm2 and a short-circuit current (Isc) of approximately 5 microA X cm-2. The effects of transport inhibitors indicated that both active Na absorption and active Cl secretion were present. Isc was increased by isoproterenol, prostaglandins E2 and F2 alpha, vasoactive intestinal peptide, dibutyryl cyclic AMP, leukotrienes C4 and D4, and bradykinin. These changes were probably due to stimulation of active Cl secretion.
犬气管黏膜细胞在培养中形成汇合的上皮片层。典型的紧密连接将细胞膜的顶端部分与基底外侧部分分隔开。细胞的顶端部分含有许多短微绒毛和明显的糖萼。除细胞间广泛的缝隙连接外，质膜的基底外侧部分没有特化。冷冻蚀刻显示培养的细胞缺乏原始组织中基底外侧的方形（正交）阵列，即先前认为与离子转运有关的颗粒。穹顶的形成表明存在活跃的液体吸收。穹顶在培养的第4至8天出现，并持续约1周。细胞片层显示跨上皮电阻约为400Ω×cm²，短路电流（Isc）约为5μA×cm⁻²。转运抑制剂的作用表明存在主动钠吸收和主动氯分泌。异丙肾上腺素、前列腺素E2和F2α、血管活性肠肽、二丁酰环磷酸腺苷、白三烯C4和D4以及缓激肽可使Isc增加。这些变化可能是由于主动氯分泌受到刺激。
