采用荧光分光光度法、液相色谱法及荧光扫描薄层色谱法测定血清中的奎尼丁。

Determination of quinidine in serum by spectrofluorometry, liquid chromatography and fluorescence scanning thin-layer chromatography.

作者信息

Vasiliades J, Finkel J M

出版信息

J Chromatogr. 1983 Nov 11;278(1):117-32. doi: 10.1016/s0378-4347(00)84762-3.

Abstract

Quinidine is determined in serum by direct and extraction spectrofluorometry, by reflectance fluorescence scanning thin-layer chromatography (TLC), and by high-performance liquid chromatography (HPLC). Least-squares analyses of patients' sera (n = 62) analyzed first by direct fluorometry (x) and then HPLC (y) gave a slope of 0.52, an y-intercept of -0.40, a standard error of estimate of 0.65, and a correlation coefficient of 0.83. Comparison of patients' sera (n = 59) determined by extraction fluorometry (x) and then HPLC (y) gave a slope of 0.998, an y-intercept of -0.175, a standard error of estimate of 0.30, and a correlation coefficient of 0.96. Comparison of patients' sera (n = 36) by HPLC (x) and then reflectance fluorescence scanning TLC (y) gave a slope of 0.837, an y-intercept of 0.152, and a correlation coefficient of 0.94. Methaqualone and oxazepam interfere with HPLC. Within-run precision is 1.6, 1.0, 5.2 and 3.0% by direct fluorometry, extraction fluorometry, TLC and HPLC while between-run precision is 5, 3.5, 9 and 6.0%, respectively.

摘要

采用直接荧光法、萃取荧光法、反射荧光扫描薄层色谱法（TLC）和高效液相色谱法（HPLC）测定血清中的奎尼丁。对62例患者血清先采用直接荧光法（x）测定，再用HPLC法（y）测定，最小二乘法分析得出斜率为0.52，y轴截距为 -0.40，估计标准误差为0.65，相关系数为0.83。对59例患者血清先采用萃取荧光法（x）测定，再用HPLC法（y）测定，结果得出斜率为0.998，y轴截距为 -0.175，估计标准误差为0.30，相关系数为0.96。对36例患者血清先采用HPLC法（x）测定，再用反射荧光扫描TLC法（y）测定，得出斜率为0.837，y轴截距为0.152，相关系数为0.94。甲喹酮和奥沙西泮会干扰HPLC测定。直接荧光法、萃取荧光法、TLC法和HPLC法的批内精密度分别为1.6%、1.0%、5.2%和3.0%，批间精密度分别为5%、3.5%、9%和6.0%。