Wesley-Hadzija B, Mattocks A M
J Chromatogr. 1977 Dec 1;144(2):223-30. doi: 10.1016/s0021-9673(00)99358-0.
A sensitive, accurate and specific spectrodensitometric method has been developed for the determination of quinidine in biological fluids. It involves extraction of quinidine, dihydroquinidine and metabolites, their separation on thin layers and quantitation of the corresponding spots by direct scanning in a densitometer at 278 nm. A linear relationship was obtained between the ratio of the peak area of an unknown sample to that of the standard and the concentration of the compounds at 0.4-4microgram/ml. The recovery from plasma was from 96 to 103% for quinidine and from 93.5 to 98.5% for dihydroquinidine. A comparison was made between this thin-layer chromatographic method and the fluoriemtric assay frequently used for the determination of quinidine in plasma at present. The method is recommended for clinical assays and pharmacokinetics studies.
已开发出一种灵敏、准确且特异的分光密度测定法，用于测定生物流体中的奎尼丁。该方法包括提取奎尼丁、二氢奎尼丁及其代谢产物，在薄层上进行分离，并通过在278nm处用密度计直接扫描对相应斑点进行定量。未知样品与标准品的峰面积比与化合物浓度在0.4 - 4微克/毫升之间呈线性关系。奎尼丁从血浆中的回收率为96%至103%，二氢奎尼丁为93.5%至98.5%。将这种薄层色谱法与目前常用于测定血浆中奎尼丁的荧光测定法进行了比较。该方法推荐用于临床分析和药代动力学研究。
