评估一种用于制备微量滴定抗生素平板并检测其储存期间效力的分配仪器（Dynatech MIC - 2000）。
Evaluation of a dispensing instrument (Dynatech MIC-2000) for preparing microtiter antibiotic plates and testing their potency during storage.
作者信息
McMaster P R, Robertson E A, Witebsky F G, MacLowry J D
出版信息
Antimicrob Agents Chemother. 1978 May;13(5):842-4. doi: 10.1128/AAC.13.5.842.
Abstract
The MIC-2000 96-channel dispenser was evaluated for accuracy and repeatability of dispensing 50-mul volumes of water. It was found to perform within the manufacturer's specifications for accuracy of +/-5%, but not within the limits for repeatability of +/-1%. Overall, instrument performance was found to be satisfactory for the antimicrobial susceptibility testing technique which has been implemented in this laboratory. A method is suggested for simple evaluation of the volumes dispensed. A variety of antibiotics may be stored in Trypticase soy broth in Microtiter plates without loss of potency for periods of at least 2 weeks at -20 degrees C and 4 months at -70 degrees C.
摘要
对MIC - 2000 96通道分配器进行了评估，以检测其分配50微升水的准确性和重复性。结果发现，其准确性在制造商规定的±5%范围内，但重复性不在±1%的限度内。总体而言，对于本实验室采用的抗菌药敏试验技术，该仪器的性能令人满意。本文提出了一种简单评估分配体积的方法。多种抗生素可储存在微量滴定板中的胰蛋白酶大豆肉汤中，在-20℃下至少可保存2周，在-70℃下可保存4个月而效力不减。
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