Plebani A, Avanzini M A, Scotta M S, Monafo V, Giunta A M, Guandalini S, Ugazio A G, Burgio R G
J Clin Lab Immunol. 1986 Jun;20(2):93-6.
An ELISA technique using labelled antigen for the determination of cow's milk specific IgE in serum is described. The use of labelled antigen, rather than labelled antibody as in the RAST, permits avoidance of interference by antibodies other than IgE, such as IgG, at times responsible for a negative RAST. The results obtained with the 2 techniques in 43 infants with a positive cow's milk challenge showed a positive RAST in 28%, a positive ELISA in 35% and a positive RAST or ELISA in 42%. These findings suggest that the use of both ELISA and RAST permits in vitro diagnosis of cow's milk allergy in more patients than either test alone.
本文描述了一种使用标记抗原的酶联免疫吸附测定（ELISA）技术，用于测定血清中牛奶特异性免疫球蛋白E（IgE）。与放射性变应原吸附试验（RAST）中使用标记抗体不同，使用标记抗原可避免非IgE抗体（如IgG）的干扰，这些抗体有时会导致RAST结果呈阴性。对43名牛奶激发试验呈阳性的婴儿采用这两种技术进行检测，结果显示，RAST阳性率为28%，ELISA阳性率为35%，RAST或ELISA阳性率为42%。这些结果表明，与单独使用任何一种检测方法相比，同时使用ELISA和RAST能够使更多患者得到牛奶过敏的体外诊断。
