Salustri A, Martinozzi M
Cell Biol Int Rep. 1983 Dec;7(12):1049-55. doi: 10.1016/0309-1651(83)90010-3.
We have studied in vitro protein synthesis in mouse denuded and follicle-enclosed oocytes by measuring 3H-leucine incorporation into TCA-precipitable material. Thirty percent more radiolabelled TCA-precipitable material was found in follicle-enclosed oocytes. Since in our experimental conditions the internal specific activity of 3H-leucine does not differ in the two systems, and no appreciable transfer of labelled protein from follicle cells to the oocyte occurs, we conclude that follicle cells have a beneficial influence on the oocyte's protein synthetic activity.
我们通过测量3H-亮氨酸掺入三氯乙酸（TCA）沉淀物质的量，研究了小鼠裸卵和卵泡包裹卵母细胞的体外蛋白质合成。在卵泡包裹的卵母细胞中发现放射性标记的TCA沉淀物质多30%。由于在我们的实验条件下，两个系统中3H-亮氨酸的内部比活性没有差异，并且没有明显的标记蛋白从卵泡细胞转移到卵母细胞，我们得出结论，卵泡细胞对卵母细胞的蛋白质合成活性有有益影响。
