Jaffe Laurinda A, Norris Rachael P, Freudzon Marina, Ratzan William J, Mehlmann Lisa M
Department of Cell Biology, University of Connecticut Health Center, Farmington, CT, USA.
Methods Mol Biol. 2009;518:157-73. doi: 10.1007/978-1-59745-202-1_12.
The mammalian oocyte develops within a complex of somatic cells known as a follicle, within which signals from the somatic cells regulate the oocyte, and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative microinjection of follicle-enclosed mouse oocytes, thus allowing the introduction of signaling molecules as well as optical probes into the oocyte within its physiological environment.
哺乳动物的卵母细胞在一种称为卵泡的体细胞复合体中发育，其中体细胞发出的信号调节卵母细胞，而卵母细胞发出的信号调节体细胞。由于将卵母细胞从卵泡中分离会破坏这些通信途径，因此最好在完整的卵泡内研究卵母细胞生理学。在这里，我们描述了对卵泡包裹的小鼠卵母细胞进行定量显微注射的方法，从而能够在其生理环境中将信号分子以及光学探针引入卵母细胞。
