Koop H U, Dirk J, Wolff D, Schweiger H G
Cell Biol Int Rep. 1983 Dec;7(12):1123-8. doi: 10.1016/0309-1651(83)90019-x.
Two single mesophyll-protoplasts of Nicotiana tabacum cv. xanthi were selected into a 100 nl microdroplet of 0.4 M mannitol. Two cylindrical platinum electrodes were inserted into the microdroplet to align the two single cells via dielectrophoresis in an AC-field (1 MHz, about 120 V X cm-1). A single square DC-pulse of about 1.5 KV X cm-1 was applied to induce protoplast fusion.
选取两个烟草品种黄花烟草的单个叶肉原生质体，放入100纳升含0.4 M甘露醇的微滴中。将两根圆柱形铂电极插入微滴中，通过交流电场（1兆赫兹，约120伏/厘米）中的介电泳使两个单细胞对齐。施加一个约1.5千伏/厘米的单个方形直流脉冲以诱导原生质体融合。
