大鼠前列腺叶间生化差异的系统研究。

A systematic study of biochemical differences between the lobes of the rat prostate.

作者信息

Gerhardt P G, Mevåg B, Tveter K J, Purvis K

出版信息

Int J Androl. 1983 Dec;6(6):553-62. doi: 10.1111/j.1365-2605.1983.tb00346.x.

DOI:10.1111/j.1365-2605.1983.tb00346.x

Abstract

The present study constitutes a systematic search for biochemical parameters which could be used to monitor the isolated function of various prostate lobes and accessory sex glands in the rat. Various parameters were measured in the 105 000 X g supernatants of the ventral, lateral and dorsal prostate lobes, and in the coagulating glands and seminal vesicles. These included spermine, citric acid, acid phosphatase, fructose and zinc, as well as the pattern of proteins in both cytosols and secretions, which were determined using one dimensional SDS, polyacrylamide gel electrophoresis. The results suggest that several parameters can be used including: Ventral lobe--spermine or prostatein; Lateral lobe--zinc or a 15 000 dalton protein; Dorsal lobe--spermine or a 60 000 dalton protein; and coagulating gland--fructose.

摘要

本研究对一系列生化参数进行了系统的探寻，这些参数可用于监测大鼠各前列腺叶及附属性腺的独立功能。对腹侧、外侧和背侧前列腺叶、凝固腺及精囊105000Xg上清液中的多种参数进行了测定。这些参数包括精胺、柠檬酸、酸性磷酸酶、果糖和锌，以及细胞质和分泌物中的蛋白质模式，后者通过一维SDS聚丙烯酰胺凝胶电泳进行测定。结果表明，可使用的参数有：腹侧叶——精胺或前列腺蛋白；外侧叶——锌或一种15000道尔顿的蛋白质；背侧叶——精胺或一种60000道尔顿的蛋白质；以及凝固腺——果糖。