Jacobs S C, Story M T
Department of Urology, Medical College of Wisconsin, Milwaukee.
Urol Res. 1989;17(5):311-5. doi: 10.1007/BF00262989.
In vivo prostatic secretion was collected from retired breeder Sprague Dawley rats using a method for isolated perfusion of the rat prostatic urethra. Enzymatic acid phosphatase determination was performed on the collected effluent. Control acid phosphatase secretion was 24.2 +/- 2.7 nm over 30 minutes. Intravenous phenylephrine 5 mg/kg stimulated a 10 fold increase in acid phosphatase secretion. The secretion seen with phenylephrine was dose dependent and could be blocked with prazosin, but not yohimbine, atropine, or propranolol. Intravenous beta-adrenergic agonist isoproterenol caused no increase in the secretion of rat prostatic acid phosphatase. Intravenous administration of the cholinergic agonist pilocarpine also resulted in a dose dependent rise in acid phosphatase secretion. The stimulation seen could be blocked by atropine but not phentolamine or propranolol. The stimulation of acid phosphatase secretion seen with alpha 1 adrenergic or cholinergic agonists was not additive. Intravenous vasoactive intestinal peptide did not stimulate acid phosphatase secretion nor did it augment the secretion induced by alpha 1 adrenergic or cholinergic agonists. Release of acid phosphatase into rat prostatic exocrine secretion is under both alpha 1 adrenergic and cholinergic control.
采用大鼠前列腺尿道分离灌注法，从退休种用斯普拉格-道利大鼠体内收集前列腺分泌物。对收集的流出液进行酸性磷酸酶活性测定。对照酸性磷酸酶分泌在30分钟内为24.2±2.7纳米。静脉注射5毫克/千克苯肾上腺素可使酸性磷酸酶分泌增加10倍。苯肾上腺素引起的分泌呈剂量依赖性，可被哌唑嗪阻断，但不能被育亨宾、阿托品或普萘洛尔阻断。静脉注射β-肾上腺素能激动剂异丙肾上腺素不会使大鼠前列腺酸性磷酸酶分泌增加。静脉注射胆碱能激动剂毛果芸香碱也会导致酸性磷酸酶分泌呈剂量依赖性增加。观察到的刺激可被阿托品阻断，但不能被酚妥拉明或普萘洛尔阻断。α1肾上腺素能或胆碱能激动剂引起的酸性磷酸酶分泌刺激无相加作用。静脉注射血管活性肠肽不会刺激酸性磷酸酶分泌，也不会增强α1肾上腺素能或胆碱能激动剂诱导的分泌。酸性磷酸酶释放到大鼠前列腺外分泌液中受α1肾上腺素能和胆碱能的双重控制。
