Haseltine W A, Maxam A M, Gilbert W
Proc Natl Acad Sci U S A. 1977 Mar;74(3):989-93. doi: 10.1073/pnas.74.3.989.
When Rous sarcoma virus RNA is transcribed into DNA by the reverse transcriptase, a tRNA primer is elongated into DNA. The primer is near the 5' end of the virus genome; the first major DNA made is a "run-off" product extending 101 bases from the primer to the 5' end of the template. We have studied this DNA molecule to determine the sequence of the first 101 bases at the 5' end of the Rous sarcoma virus genome (Prague strain, subgroup C). Twenty-one bases at the extreme 5' end are also at the 3' end of the virus genome (see D. E. Schwartz, P. C. Zamecnik, and H. L. Weith, this issue, pp. 994-998), and thus this virus is terminally redundant. The existence of this sequence repetition immediately suggests mechanisms by which the growing DNA copy can jump from the 5' end to a 3' end of the template and become circular. The sequence also displays a possible ribosome binding site and enough secondary structure to permit a possible 5'-5' linkage of viral RNA molecules.
当劳氏肉瘤病毒RNA通过逆转录酶转录成DNA时，一个tRNA引物被延长成DNA。该引物靠近病毒基因组的5'端；合成的第一个主要DNA是一种“延伸产物”，从引物延伸101个碱基至模板的5'端。我们研究了这个DNA分子，以确定劳氏肉瘤病毒基因组（布拉格株，C亚组）5'端前101个碱基的序列。最末端5'端的21个碱基也位于病毒基因组的3'端（见D.E.施瓦茨、P.C.扎梅尼克和H.L.魏思，本期，第994 - 998页），因此这种病毒是末端冗余的。这种序列重复的存在立即提示了正在增长的DNA拷贝从模板的5'端跳跃到3'端并形成环状的机制。该序列还显示出一个可能的核糖体结合位点以及足够的二级结构，以允许病毒RNA分子可能的5'-5'连接。
