Stephenson M L, Zamecnik P C
Proc Natl Acad Sci U S A. 1978 Jan;75(1):285-8. doi: 10.1073/pnas.75.1.285.
A tridecamer oligodeoxynucleotide, d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to reiterated 3'- and 5'-terminal nucleotides of Rous sarcoma virus 35S RNA, is an efficient inhibitor of the translation of proteins specified by the viral RNA in the wheat embryo cell-free system. The inhibition specificity for oncornavirus RNA is greater than for rabbit reticulocyte mRNA or brome mosaic virus RNA. Other oligodeoxynucleotides of similar size have little or no specific effect on the RNA-directed translation. The tridecamer acts as a primer for the avian myeloblastosis virus DNA polymerase when Rous sarcoma virus heated 70S RNA is used as a template, offering evidence that it can hybridize to the RNA. The possible use of such an oligodeoxynucleotide hybridization competitor to inhibit Rous sarcoma virus replication is described in the preceding paper [Zamecnik, P. C. & Stephenson, M. L. (1978) Proc. Natl. Acad. Sci. USA. 75, 280--284].
一种十三聚体寡脱氧核苷酸d(A - A - T - G - G - T - A - A - A - A - T - G - G)，它与劳氏肉瘤病毒35S RNA的重复3'端和5'端核苷酸互补，在小麦胚无细胞系统中是病毒RNA所编码蛋白质翻译的有效抑制剂。对肿瘤病毒RNA的抑制特异性大于对兔网织红细胞mRNA或雀麦花叶病毒RNA的抑制特异性。其他大小相似的寡脱氧核苷酸对RNA指导的翻译几乎没有或没有特异性作用。当以加热的劳氏肉瘤病毒70S RNA为模板时，该十三聚体可作为禽成髓细胞瘤病毒DNA聚合酶的引物，这表明它能与RNA杂交。前文[扎梅尼克，P. C. & 斯蒂芬森，M. L. (1978) 《美国国家科学院院刊》75, 280 - 284]描述了这种寡脱氧核苷酸杂交竞争剂抑制劳氏肉瘤病毒复制的可能用途。
