Dexter T M, Spooncer E, Toksoz D, Lajtha L G
J Supramol Struct. 1980;13(4):513-24. doi: 10.1002/jss.400130410.
In long-term marrow cultures haemopoiesis can be maintained in vitro for up to 6 months. Critical analysis of the cell populations produced has shown that the stem cells and their committed progeny have characteristics in common with the corresponding cell types in vivo. The maintenance of haemopoiesis in vitro is associated with the development of an appropriate inductive environment provided by bone marrow derived adherent cells. Analysis of the interactions between environmental and haemopoietic cells has been facilitated by the development of vitro systems reproducing the naturally occurring genetic environmental defects and other systems where the development of a competent inductive environment shows a dependency upon corticosteroid hormones. Investigations have shown that stem cell proliferation may be controlled by production of opposing activities, one stimulatory for DNA synthesis, the other inhibitory. A model is proposed whereby modulation in the production of these factors is determined by the physical presence of stem cells in a proposed cellular milieu, within the adherent layer. The adherent layer, apart from acting at the level of stem cell proliferation, can also modify the response of differentiating cells (eg, GM-CFC) to exogenous stimulatory activities. Addition of GM-CSF or of CSF-antiserum has no effect on haemopoiesis in long-term cultures.
在长期骨髓培养中，造血功能可在体外维持长达6个月。对所产生的细胞群体进行的严格分析表明，干细胞及其定向子代细胞具有与体内相应细胞类型相同的特征。体外造血功能的维持与骨髓来源的贴壁细胞提供的适当诱导环境的发育有关。通过开发再现自然发生的遗传环境缺陷的体外系统以及其他有能力的诱导环境的发育依赖于皮质类固醇激素的系统，促进了对环境细胞与造血细胞之间相互作用的分析。研究表明，干细胞增殖可能受相反活性产物的控制，一种刺激DNA合成，另一种抑制DNA合成。提出了一个模型，据此这些因子产生的调节由贴壁层中假定细胞环境中干细胞的实际存在决定。贴壁层除了在干细胞增殖水平起作用外，还可以改变分化细胞（如GM-CFC）对外源刺激活性的反应。添加GM-CSF或CSF抗血清对长期培养中的造血功能没有影响。
