Kroon A M, de Vos W M, Bakker H
Biochim Biophys Acta. 1978 Jun 22;519(1):269-73. doi: 10.1016/0005-2787(78)90079-5.
Two types of mitochondrial DNA (mtDNA) can be distinquished in an inbred strain of rats of the Wistar type. The population of DNA molecules of the liver of one single rat is homogeneous. This was shown for a number of 100 animals and confirms the data of other investigators. The two types of mitochondrial DNA, designated A and B, differ in their number of cleavage sites for the restriction endonucleases Eco RI (2sites), Hind II (1 site) and Hha I (1 site). No differences were found for the restriction enzymes Bam HI, Hap II, Hind III and Hpa I. The degree of sequence divergence of the two types of DNA is calculated to be roughly 5% on the basis of these observations. From 20 rats part of the liver was taken and the mtDNA was characterized. Heterologous and homologous crosses between type A and type B rats were made. Analysis of the offspring revealed strictly maternal inheritance of the A and B mtDNA traits. For purposes of base-sequence analysis and RNA.DNA hybridization the strain could easily be "purified" genetically.
在近交系Wistar大鼠中可区分出两种类型的线粒体DNA（mtDNA）。一只大鼠肝脏中的DNA分子群体是同质的。对100只动物进行的研究表明了这一点，并证实了其他研究者的数据。这两种类型的线粒体DNA，分别命名为A和B，在限制性内切酶Eco RI（2个位点）、Hind II（1个位点）和Hha I（1个位点）的切割位点数量上有所不同。对于限制性酶Bam HI、Hap II、Hind III和Hpa I未发现差异。基于这些观察结果，计算出这两种类型DNA的序列分歧程度约为5%。从20只大鼠身上取部分肝脏并对mtDNA进行了表征。进行了A类和B类大鼠之间的异源和同源杂交。对后代的分析揭示了A和B mtDNA性状的严格母系遗传。为了进行碱基序列分析和RNA-DNA杂交，该品系可以很容易地在遗传上“纯化”。
