Luthe D S
Anal Biochem. 1983 Nov;135(1):230-2. doi: 10.1016/0003-2697(83)90755-8.
A method for preparing multiple sucrose gradients by quickly freezing layers of sucrose has been developed. These gradients may be stored in the freezer indefinitely, and thawed from 8 to 24 h at 4 degrees C before use. The middle region of the resulting sucrose gradients was linear. Thawing time and centrifugation had little effect on the shape of the gradient. The method is applicable for both small- and large-volume centrifuge tubes. Gradients prepared in the same batch were nearly identical.
一种通过快速冷冻蔗糖层来制备多个蔗糖梯度的方法已被开发出来。这些梯度可以无限期地保存在冰箱中，并在使用前于4摄氏度下解冻8至24小时。所得蔗糖梯度的中间区域呈线性。解冻时间和离心对梯度形状影响很小。该方法适用于小体积和大体积的离心管。同一批次制备的梯度几乎相同。
