Ishiwata S
Biochim Biophys Acta. 1978 Jun 21;534(2):350-7. doi: 10.1016/0005-2795(78)90018-1.
We studied the effects of the troponin components TN-T, TN-I and TN-C, and of Ca2+ concentration on the dissociation temperature of the F-actin.tropomyosin complex. Troponin components and their complexes increased the dissociation temperature in the following sequence of effectiveness: TN-C(+/- Ca2+) less than TN-I less than TN-I.TN-C(+/-Ca2+) less than TN-T.TN-C(+/- Ca2+) less than TN-T.TN-C(--Ca2+) approximately TN-T less than TN-T.TN-I.TN-C(+Ca2+) less than TN-T.TN-I.TN-C(--Ca2+) approximately TN-T.TN-I; TN-C has no effect. The effect of TN-I is small. TN-T is the most important component for increasing the dissociation temperature. The shifting by Ca2+ of the dissociation temperature to lower values is only seen when both TN-T and TN-C are present.
我们研究了肌钙蛋白组分TN-T、TN-I和TN-C以及钙离子浓度对F-肌动蛋白-原肌球蛋白复合物解离温度的影响。肌钙蛋白组分及其复合物按照以下有效性顺序提高解离温度：TN-C(+/- Ca2+) ＜ TN-I ＜ TN-I.TN-C(+/-Ca2+) ＜ TN-T.TN-C(+/- Ca2+) ＜ TN-T.TN-C(--Ca2+) ≈ TN-T ＜ TN-T.TN-I.TN-C(+Ca2+) ＜ TN-T.TN-I.TN-C(--Ca2+) ≈ TN-T.TN-I；TN-C无作用。TN-I的作用较小。TN-T是提高解离温度最重要的组分。只有当TN-T和TN-C都存在时，才会出现钙离子使解离温度向更低值的转变。
