Chen S S, Kou A Y, Chen H H
J Chromatogr. 1983 Aug 12;276(1):37-44. doi: 10.1016/s0378-4347(00)85063-x.
A simple and rapid high-performance liquid chromatographic procedure for the quantitative analysis of ethanolamine- and serine-containing phospholipids in tissue is described. The technique involves reaction of lipid extracts with succinimidyl 2-naphthoxyacetate to give fluorescent derivatives of aminophospholipids. Reaction products are separated by a silica gel column with gradient elution. The eluate is monitored by fluorescence detection at 228 nm (excitation) and 342 nm (emission). Ethanolamine and serine plasmalogens can be measured indirectly by converting their derivatives into lysophosphatidylethanolamine and lysophosphatidylserine derivatives with exposure to hydrogen chloride fumes. The method is highly sensitive and selective.
描述了一种用于组织中含乙醇胺和丝氨酸的磷脂定量分析的简单快速高效液相色谱方法。该技术涉及脂质提取物与2-萘氧基乙酸琥珀酰亚胺酯反应，生成氨基磷脂的荧光衍生物。反应产物通过硅胶柱梯度洗脱进行分离。洗脱液通过在228nm（激发）和342nm（发射）处的荧光检测进行监测。乙醇胺和丝氨酸缩醛磷脂可通过将其衍生物暴露于氯化氢气体中转化为溶血磷脂酰乙醇胺和溶血磷脂酰丝氨酸衍生物来间接测定。该方法具有高灵敏度和选择性。
