高效液相色谱法分离磷脂的改进方法
Improved procedure for the separation of phospholipids by high-performance liquid chromatography.
作者信息
Chen S S, Kou A Y
出版信息
J Chromatogr. 1982 Jan 8;227(1):25-31. doi: 10.1016/s0378-4347(00)80352-7.
We describe a rapid and efficient high-performance liquid chromatography procedure for the separation of phospholipids. The separation is accomplished on a microparticulate silica gel column using isocratic elution and UV detection at 203 nm. The solvent mixture is acetonitrile--methanol--85% phosphoric acid(130:5:1.5, v/v). Complete separation is achieved within 30 min of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, lysophosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine and sphingomyelin. The method is suitable for the analysis of phospholipids in tissue extracts.
我们描述了一种用于分离磷脂的快速高效的高效液相色谱方法。该分离在微粒硅胶柱上进行，采用等度洗脱，并在203nm处进行紫外检测。溶剂混合物为乙腈 - 甲醇 - 85%磷酸（130:5:1.5，v/v）。在30分钟内可实现磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰乙醇胺、磷脂酰二甲基乙醇胺、溶血磷脂酰乙醇胺、磷脂酰胆碱、溶血磷脂酰胆碱和鞘磷脂的完全分离。该方法适用于组织提取物中磷脂的分析。
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