Tornqvist H, Jeppsson J O, Nilsson K O
Acta Paediatr Scand. 1983 Nov;72(6):911-5. doi: 10.1111/j.1651-2227.1983.tb09839.x.
Day-to-day fluctuations in blood concentration of the glycosylated hemoglobin, HbA1c or HbA1, are mainly due to variations in content of the intermediate aldimide form, preHbA1c. Using electrofocusing we have studied this component adjacent to the HbA1c band, which represents the stable ketoamine form. The fraction of preHbA1c present rapidly increases upon incubation of erythrocytes in 20 mM glucose at +37 degrees C, and the reaction is fully reversible if glucose is removed. In children with insulin-dependent diabetes mellitus with different degrees of metabolic control, levels of preHbA1c varied between 0.2 and 3.7% of total hemoglobin (median value 1.3%, N = 25). A clear correlation was found between preHbA1c and urinary glucose concentrations during the 12 hours preceding blood sampling (r = 0.75, p less than 0.001, df = 18). As expected, pre-HbA1c did not correlate to HbA1c or glycosylated albumin, which reflect long- and medium-term diabetic control.
糖化血红蛋白（HbA1c或HbA1）的血液浓度每日波动主要归因于中间醛亚胺形式即前HbA1c含量的变化。我们使用电聚焦法研究了HbA1c条带附近的这一成分，HbA1c条带代表稳定的酮胺形式。将红细胞在37℃的20 mM葡萄糖中孵育时，前HbA1c的比例迅速增加，若去除葡萄糖，该反应完全可逆。在不同代谢控制程度的胰岛素依赖型糖尿病儿童中，前HbA1c水平在总血红蛋白的0.2%至3.7%之间变化（中位数为1.3%，N = 25）。在采血前12小时内，前HbA1c与尿葡萄糖浓度之间存在明显相关性（r = 0.75，p < 0.001，自由度 = 18）。正如预期的那样，前HbA1c与反映长期和中期糖尿病控制情况的HbA1c或糖化白蛋白无相关性。
