Gahmberg C G, Jokinen M, Andersson L C
Blood. 1978 Aug;52(2):379-87.
The major sialoglycoprotein of human erythrocyte membranes (glycophorin) is one of the most-studied membrane proteins. Although the structure is relatively well known, almost nothing is known about its expression in erythroid cells. To study this we raised an antiserum that reacted specifically with this protein. This was accomplished by immunization of rabbits with a preparation of glycophorin followed by absorption with En(a-) erythrocyte membranes, which lack glycophorin. By use of this antiserum and a staphylococcus protein A technique we could establish that only bone marrow cells of erythrocyte lineage express glycophorin at the cell surface. This occurs in basophilic normoblasts and later stages of erythrocyte differentiation, whereas pronormoblasts do not seem to contain glycophorin.
人类红细胞膜的主要唾液糖蛋白（血型糖蛋白）是研究最为深入的膜蛋白之一。尽管其结构已相对明确，但对于它在红系细胞中的表达情况却几乎一无所知。为了研究这一点，我们制备了一种能与该蛋白特异性反应的抗血清。这是通过用血型糖蛋白制剂免疫兔子，然后用缺乏血型糖蛋白的恩（a-）红细胞膜进行吸收来实现的。利用这种抗血清和葡萄球菌蛋白A技术，我们能够确定只有红细胞系的骨髓细胞在细胞表面表达血型糖蛋白。这种情况发生在嗜碱性正成红细胞及红细胞分化的后期阶段，而原成红细胞似乎不含有血型糖蛋白。
