Tsuda H, Dickey W D, Goldman A S
Cell Struct Funct. 1983 Dec;8(4):367-71. doi: 10.1247/csf.8.367.
A new technique for separation of human colostral macrophages and neutrophils was developed. Macrophages attached more readily to acid soluble collagen-serum or gelatin-serum substrata than neutrophils. Most of the adherent neutrophils were removed during the first 5 minutes incubation in 3 mM EDTA, thereafter, complete detachment of macrophages followed. Neutrophils and macrophages were enriched more than 80% in nonadherent cell populations and detached cell populations, respectively. These separated colostral leukocytes retained their viability and phagocytic activities. Therefore, functional studies of these purified human colostral leukocytes are possible.
开发了一种分离人初乳巨噬细胞和中性粒细胞的新技术。巨噬细胞比中性粒细胞更容易附着于酸溶性胶原-血清或明胶-血清基质。在3 mM EDTA中孵育的前5分钟，大多数黏附的中性粒细胞被去除，此后，巨噬细胞完全脱离。中性粒细胞和巨噬细胞分别在非黏附细胞群体和脱离细胞群体中富集超过80%。这些分离的初乳白细胞保持了它们的活力和吞噬活性。因此，对这些纯化的人初乳白细胞进行功能研究是可行的。
