Chan S Y, Tang L C, Tang G W, Chan P H
Contraception. 1983 Nov;28(5):481-8. doi: 10.1016/0010-7824(83)90079-3.
The potential functions of testosterone and 5 alpha-dihydrotestosterone, the androgens normally present in human seminal plasma, on human spermatozoal physiology were evaluated by studying the effects of these two steroid hormones on the in vitro fertilizing capacity of human spermatozoa. Spermatozoa collected from presumably fertile men were washed in BWW medium and incubated with different concentrations (0, 100, 250, 500, 1000 pg/ml) of testosterone or 5 alpha-dihydrotestosterone for 5 hr before insemination of the zona-free hamster ova. Penetration of the zona-free hamster ova was scored 6 hr later and the results were analyzed statistically. Both testosterone and 5 alpha-dihydrotestosterone, at the concentrations tested, significantly decreased the in vitro penetration of the denuded hamster ova in comparison to the controls (p less than 0.05). A dose-dependent response was also observed for the 5 alpha-dihydrotestosterone tested. These findings indicate that exogenous testosterone and 5 alpha-dihydrotestosterone can inhibit the fertilizing capacity of human spermatozoa in vitro, and suggest that the androgens normally present in human seminal plasma may serve, in part, to prevent premature spermatozoal capacitation before the spermatozoa reach the site of fertilization in vivo.
通过研究这两种甾体激素对人类精子体外受精能力的影响，评估了睾酮和5α-双氢睾酮（通常存在于人类精浆中的雄激素）对人类精子生理的潜在作用。从可能具有生育能力的男性中收集的精子在BWW培养基中洗涤，并在授精无透明带仓鼠卵前，与不同浓度（0、100、250、500、1000 pg/ml）的睾酮或5α-双氢睾酮孵育5小时。6小时后对无透明带仓鼠卵的穿透情况进行评分，并对结果进行统计学分析。与对照组相比，在所测试的浓度下，睾酮和5α-双氢睾酮均显著降低了裸仓鼠卵的体外穿透率（p<0.05）。在所测试的5α-双氢睾酮中也观察到了剂量依赖性反应。这些发现表明，外源性睾酮和5α-双氢睾酮可在体外抑制人类精子的受精能力，并提示正常存在于人类精浆中的雄激素可能在一定程度上有助于防止精子在体内到达受精部位之前过早发生获能。
