Chan S Y
Cell Biol Int Rep. 1985 Feb;9(2):127-30. doi: 10.1016/0309-1651(85)90086-4.
The effect of taurine at various concentrations (0.01, 0.1 and 1 mM) on the in vitro motility and fertilizing capacity of human spermatozoa was studied. Spermatozoa collected from 10 normal men were washed in BWW medium and incubated with taurine for 5 hours, the period required for spermatozoal capacitation. The percent motilities were recorded at 0 and 5 hours during capacitation preincubation with taurine. After incubation, the spermatozoa were washed with BWW medium to remove taurine before insemination of the zona-free hamster ova for an assessment of the fertilizing capacity. Taurine caused a significant dose-dependent increase in the penetration of the zona-free hamster ova in comparison to the control (p less than 0.05). Taurine did not have any significant effects on the spermatozoal motility during capacitation preincubation. The results suggest that there may be a physiological role for this beta-amino acid in human spermatozoal capacitation in vivo.
研究了不同浓度（0.01、0.1和1 mM）的牛磺酸对人精子体外活力和受精能力的影响。从10名正常男性收集的精子在BWW培养基中洗涤，并与牛磺酸孵育5小时，这是精子获能所需的时间。在与牛磺酸进行获能预孵育的0小时和5小时记录活力百分比。孵育后，在将精子用于无透明带仓鼠卵授精以评估受精能力之前，用BWW培养基洗涤以去除牛磺酸。与对照组相比，牛磺酸导致无透明带仓鼠卵的穿透率显著呈剂量依赖性增加（p小于0.05）。在获能预孵育期间，牛磺酸对精子活力没有任何显著影响。结果表明，这种β-氨基酸在体内人精子获能过程中可能具有生理作用。
