Ohno N, Matsumoto S, Suzuki I, Miyazaki T, Kumazawa Y, Otsuka Y, Yadomae T
J Pharmacobiodyn. 1983 Dec;6(12):903-12. doi: 10.1248/bpb1978.6.903.
Chemical composition and physicochemical properties of an immunomodulator, which is a non-dialyzable and acetone precipitable material(s) extracted with hot water from Angelica actiloba KITAGAWA (Yamato Tohki) (AIP), were investigated. AIP was composed of about 90% sugar and 10% protein. The major polysaccharide was identified as pectic substance(s) because its main component sugars were found to be arabinose, glucose, and galacturonic acid by gas liquid chromatographic analysis. The pectic substance(s) was not concerned with the mitogenicity of AIP since the activity was similar before and after pectinase(endo-polygalacturonase) treatment. More than half of the mitogenicity was destroyed by acid or alkali treatment. With pronase treatment, the activity was not affected, but the molecular weight of the mitogen was lowered. In addition, the mitogenic substance was partially purified from AIP by pectinase treatment and Westphal's phenol/water fractionation. The partially purified mitogenic substance(s) was rich in protein. These facts suggest that the mitogenicity of AIP was carried by a heat stable and protease resistant protein.
对一种免疫调节剂的化学成分和理化性质进行了研究。该免疫调节剂是从当归（大和独活）中用热水提取的一种不可透析且可被丙酮沉淀的物质（AIP）。AIP由约90%的糖和10%的蛋白质组成。通过气液色谱分析发现其主要成分糖为阿拉伯糖、葡萄糖和半乳糖醛酸，因此主要多糖被鉴定为果胶物质。果胶物质与AIP的促有丝分裂活性无关，因为果胶酶（内切多聚半乳糖醛酸酶）处理前后活性相似。超过一半的促有丝分裂活性被酸或碱处理破坏。用链霉蛋白酶处理后，活性不受影响，但促有丝分裂原的分子量降低。此外，通过果胶酶处理和韦斯特法尔酚/水分级分离从AIP中部分纯化了促有丝分裂物质。部分纯化的促有丝分裂物质富含蛋白质。这些事实表明，AIP的促有丝分裂活性由一种热稳定且抗蛋白酶的蛋白质承载。
