Oka H, Ohno N, Iwanaga S, Izumi S, Kawakita T, Nomoto K, Yadomae T
Laboratory of Immunopharmacology of Microbial Products, Tokoyo University of Pharmacy and Life Science, Japan.
Biol Pharm Bull. 1995 May;18(5):757-65. doi: 10.1248/bpb.18.757.
Bupleuri Radix is a commonly used medicinal plant in Kampo medicine, and its hot water extracts show mitogenic activity to murine lymphocytes. In this paper the mitogenic substances in the hot water extracts of Bupleuri Radix (Bup-HWE) were fractionated and characterized physicochemically and immunologically. Most of these substances were recovered from mol. wt of more than 200 kDA fraction (fr. C-13). Separation of fr. C-13 by phenol-water fractionation method gave water soluble and phenol soluble mitogenic substances. These substances showed the activity even in C3H/HeJ mice, and polymyxin B or lysozyme treatment did not abrogate the activity, suggesting that the active substances are not related to bacterial lipopolysaccharide. Treatment of the mitogenic substances recovered from the phenol layer with NaCLO2, a polyphenol degrading chemical, significantly reduced the activity, but pronase and pectinase treatments were not effective. The mitogenic substances in the water layer were active even after NaCLO2 treatment. These findings suggested that the mitogenic substances of Bup-HWE are large molecular weight polyphenolic compounds and polysaccharide. The mitogenic substances are suggested to be B cell mitogens.
柴胡是汉方医学中常用的药用植物，其热水提取物对小鼠淋巴细胞具有促有丝分裂活性。本文对柴胡热水提取物（Bup-HWE）中的促有丝分裂物质进行了分离，并对其进行了物理化学和免疫学表征。这些物质大多从分子量超过200 kDa的组分（组分C-13）中回收。通过酚-水分离法对组分C-13进行分离，得到了水溶性和酚溶性的促有丝分裂物质。这些物质在C3H/HeJ小鼠中也表现出活性，并且多粘菌素B或溶菌酶处理并未消除该活性，这表明活性物质与细菌脂多糖无关。用多酚降解化学物质NaCLO2处理从酚层中回收的促有丝分裂物质，活性显著降低，但蛋白酶和果胶酶处理无效。水层中的促有丝分裂物质在NaCLO2处理后仍具有活性。这些发现表明，Bup-HWE的促有丝分裂物质是大分子多酚化合物和多糖。促有丝分裂物质被认为是B细胞促有丝分裂原。
