Ohno N, Suzuki I, Miyazaki T, Yadomae T
Microbiol Immunol. 1984;28(7):821-30. doi: 10.1111/j.1348-0421.1984.tb00737.x.
Active site(s) of a B-cell mitogen, vesiculogen, was investigated by means of chemical and enzymic modifications. Vesiculogen is a non-dialyzable fraction of a hot water extract from a fungus, Peziza vesiculosa (Yadomae et al, Microbiol. Immunol. 23, 997 (1979], and was composed of protein (approximately 60%), carbohydrate (approximately 30%), and a small amount of amino sugar, uronic acid, phosphate, and lipid. The mitogenicity was not affected by periodate oxidation, N-acylation, defatting treatment, destruction of the three dimensional structure, protease digestion, nuclease digestion, N-bromosuccinimide treatment, and beta-mercaptoethanol treatment. However, the mitogenicity was decreased by a modification of carboxyl groups, such as 1-ethyl-3(3-dimethylamino propyl)-carbodiimide or dicyclohexylcarbodiimide treatment. Vesiculogen contains a large amount of acidic amino acids. These results suggest that the mitogenicity of vesiculogen is due to the presence of anionic groups, such as aspartic acid and glutamic acid, and that the mitogenic substance is a novel, heat stable polyanionic B-cell activator obtained from Ascomycotina.
通过化学修饰和酶促修饰手段，对B细胞促有丝分裂原泡囊生成素的活性位点进行了研究。泡囊生成素是从真菌水泡状盘菌（矢间等，《微生物学与免疫学》23，997 [1979]）热水提取物中不可透析的部分，由蛋白质（约60%）、碳水化合物（约30%）以及少量氨基糖、糖醛酸、磷酸盐和脂质组成。其促有丝分裂活性不受高碘酸盐氧化、N - 酰化、脱脂处理、三维结构破坏、蛋白酶消化、核酸酶消化、N - 溴代琥珀酰亚胺处理和β - 巯基乙醇处理的影响。然而，通过羧基修饰，如1 - 乙基 - 3（3 - 二甲基氨基丙基）- 碳二亚胺或二环己基碳二亚胺处理，其促有丝分裂活性会降低。泡囊生成素含有大量酸性氨基酸。这些结果表明，泡囊生成素的促有丝分裂活性归因于天冬氨酸和谷氨酸等阴离子基团的存在，且该促有丝分裂物质是一种从子囊菌纲获得的新型、热稳定的多阴离子B细胞激活剂。
