Ascenzi A, Bonucci E, Ripamonti A, Roveri N
Calcif Tissue Res. 1978 May 26;25(2):133-43. doi: 10.1007/BF02010762.
To obtain information on the changes in the inorganic bone fraction during calcification, low- and wide-angle X-ray diffraction techniques and electron microscopy have been applied to single osteon samples. The samples were cylindrically shaped and their axes corresponded to the axes of the Haversian canals. The selection was made according to the degree of calcification and the orientation of collagen bundles and inorganic particles. Osteons at both the initial and final stages of calcification were chosen. Arrangements of fiber bundles and inorganic particles in successive lamellae characteristic of three types of osteon were selected, that is, longitudinally structured osteons, transversely structured osteons, and alternately structured osteons. The results indicate that in osteonic lamellar bone there are two types of inorganic particles: (1) granules arranged in linear or needle-shapred entities with maximum width 40-45 A, which are regularly distributed at the level of the main band of the collagen fibrils where their maximum length reaches the length of the main band itself; that is , about 400 A; and (2) very long crystallites, with a diameter of 40-45 A, which grow with their crystallographic c-axis parallel to the collagen fibrils and cover much more than a major collagen period.
为了获取钙化过程中无机骨部分的变化信息，已将低角度和广角X射线衍射技术以及电子显微镜应用于单个骨单位样本。样本呈圆柱形，其轴与哈弗斯管的轴相对应。根据钙化程度以及胶原束和无机颗粒的取向进行选择。选择了钙化初始阶段和最终阶段的骨单位。选取了三种类型骨单位连续薄片中纤维束和无机颗粒的排列方式，即纵向结构的骨单位、横向结构的骨单位和交替结构的骨单位。结果表明，在骨单位层状骨中有两种类型的无机颗粒：（1）排列成线性或针状实体的颗粒，最大宽度为40 - 45埃，它们规则地分布在胶原纤维主带水平，其最大长度达到主带本身的长度，即约400埃；（2）非常长的微晶，直径为40 - 45埃，其晶体学c轴平行于胶原纤维生长，覆盖的范围远远超过一个主要胶原周期。
