Tanemura H, Saji S, Takekoshi T, Yamamoto S, Oshita H, Sakata K
Nihon Geka Gakkai Zasshi. 1983 Sep;84(9):982-6.
In view of popularity of splenectomy in combination with gastrectomy for gastric cancer, immunological effect of splenectomy was studied experimentally, using SD rats and 3-methylcholanthrene-induced mammary cancer, MRMT -1. Splenectomy was performed on day -14, 2, 7, 14 or 21 of sc inoculation of 200 mg (about 4 X 10(6) cells) of MRMT -1 at the back of 4-week-old female rats. Tumor growth and immunocompetence of peripheral lymphocytes, spleen cells and thymus cells were examined. Tumor growth on day 35 tended to be inhibited in rats splenectomized on day -14, 2, or 14, while it tended to be promoted in rats splenectomized on day 7. PHA-induced blastogenesis and NK activity of spleen cells were reduced on day 2, rose on day 7 and gradually decreased on days 14 and 21. Winn's neutralization assay revealed that spleen cells on days 2, 14 and 21 of tumor inoculation had suppressor activities. In conclusion, spleen cells of tumor-bearing rats showed a reduced immunocompetence on day 2 (the early tumor-bearing stage) and on day 14 and later (the late tumor-bearing stage), while they showed a raised immunocompetence on day 7 (the middle tumor-bearing period).
鉴于脾切除术联合胃癌胃切除术的普及，利用SD大鼠和3-甲基胆蒽诱导的乳腺癌MRMT -1对脾切除术的免疫效应进行了实验研究。在4周龄雌性大鼠背部皮下接种200 mg（约4×10⁶个细胞）MRMT -1后的第-14、2、7、14或21天进行脾切除术。检测肿瘤生长以及外周淋巴细胞、脾细胞和胸腺细胞的免疫能力。在第-14、2或14天接受脾切除术的大鼠中，第35天的肿瘤生长趋于受到抑制，而在第7天接受脾切除术的大鼠中，肿瘤生长趋于被促进。脾细胞的PHA诱导的母细胞化和NK活性在第2天降低，在第7天升高，并在第14天和第21天逐渐下降。温氏中和试验显示，在肿瘤接种后的第2、14和21天，脾细胞具有抑制活性。总之，荷瘤大鼠的脾细胞在第2天（荷瘤早期）和第14天及以后（荷瘤晚期）免疫能力降低，而在第7天（荷瘤中期）免疫能力升高。
