一种用于检测经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的纳克量蛋白质的微量放射性碘化方法。

A microradioiodination method for detection of nanogram amounts of proteins resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

作者信息

Krakow J S, Scheinberg D A, Strand M

出版信息

J Virol Methods. 1983 Dec;7(5-6):273-8. doi: 10.1016/0166-0934(83)90079-4.

Abstract

A procedure is described for the rapid analysis of nanogram quantities of protein resolved by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). Proteins are first radioiodinated by the chloramine T method, acid precipitated after addition of a visible marker protein and collected on a nitrocellulose filter. The region of the filter containing protein is excised and the proteins are then extracted from the filter into electrophoresis sample buffer.

摘要

本文描述了一种用于快速分析经十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）分离的纳克级蛋白质的方法。蛋白质首先通过氯胺T法进行放射性碘化，加入可见标记蛋白后进行酸沉淀，并收集在硝酸纤维素滤膜上。将滤膜上含有蛋白质的区域切下，然后将蛋白质从滤膜中提取到电泳样品缓冲液中。