Poiley J A, Ernst M K, Cavanaugh D M, Raineri R
J Appl Toxicol. 1983 Dec;3(6):326-31. doi: 10.1002/jat.2550030611.
N-2-Acetylaminofluorene (AAF), a potent carcinogen in a variety of animal species and organs, was used to determine the metabolic capabilities of isolated organ cells in transformation as well as biochemical studies. Cells isolated from liver, lung, small intestine, kidney and bladder were compared with hamster embryo fibroblasts (target cells in the transformation studies) and rat mammary fibroblasts in all studies. In addition to studying AAF activation by the cells, we also determined the levels of whole-cell binding. Liver, kidney, small intestine and lung cells from hamsters, and liver, kidney and lung cells from rats showed high levels of AAF metabolism to 2-aminofluorene and N-hydroxy-2-acetylaminofluorene. The highest levels of covalent binding to intact cells were seen with the same cell types. These cells were also effective in activating AAF to a form which transformed hamster embryo cells. Cells isolated from a variety of organs can activate AAF as evidenced by the metabolites which are formed and by the levels of whole cell binding. Furthermore, hamster embryo cells are transformed when co-incubated with a variety of organ cells and AAF.
N-2-乙酰氨基芴（AAF）是多种动物物种和器官中的一种强效致癌物，被用于确定分离的器官细胞在转化方面的代谢能力以及进行生化研究。在所有研究中，将从肝脏、肺、小肠、肾脏和膀胱分离的细胞与仓鼠胚胎成纤维细胞（转化研究中的靶细胞）和大鼠乳腺成纤维细胞进行比较。除了研究细胞对AAF的激活作用外，我们还测定了全细胞结合水平。仓鼠的肝脏、肾脏、小肠和肺细胞以及大鼠的肝脏、肾脏和肺细胞对AAF代谢生成2-氨基芴和N-羟基-2-乙酰氨基芴的水平较高。相同细胞类型与完整细胞的共价结合水平最高。这些细胞还能有效地将AAF激活为一种可转化仓鼠胚胎细胞的形式。从多种器官分离的细胞可以激活AAF，这一点可通过形成的代谢产物和全细胞结合水平得到证明。此外，当仓鼠胚胎细胞与多种器官细胞和AAF共同孵育时会发生转化。
