Cicchella G, Baldoni V, Bandinelli R, Bavazzano P, Caldini G, Gori R
Quad Sclavo Diagn. 1983 Sep;19(3):301-12.
Results of an interlaboratory precision comparison programme of blood lead determination are presented. The samples consisted of bovine haemolyzed whole blood to which were added in pool small quantities of lead nitrate aqueous solutions to obtain two different concentrations of the metal (i.e. about 30 and 70 micrograms/dl). After each pool was well mixed it was divided into 15 ml portions in screw-top linear polyethylene vials and stored at -25 degrees C. The analytical methods used by the five public health laboratories participating in the programme were based on atomic absorption spectrophotometry. Three laboratories used methods which incorporate discrete sampling techniques (i.e. tantalum boat, electrothermal atomisation) and the remaining two, a method incorporating a sample preconcentration by solvent extraction and flame atomisation. During the six months period of the programme, fifty five results were obtained for each of the two pools of blood. Common statistical methods and the graphic system devised by Youden were used for data analysis. An acceptable agreement among results from the five laboratories has been obtained. The usefulness of this type of programme in improving analytical proficiency has been confirmed.
本文介绍了一项血铅测定实验室间精密度比对计划的结果。样本为牛溶血全血，向其中集中加入少量硝酸铅水溶液，以获得两种不同浓度的金属（即约30和70微克/分升）。每个样本池充分混合后，在带螺旋盖的线性聚乙烯小瓶中分成15毫升的部分，并储存在-25℃下。参与该计划的五个公共卫生实验室使用的分析方法基于原子吸收分光光度法。三个实验室使用了包含离散采样技术的方法（即钽舟、电热原子化），其余两个实验室使用了一种通过溶剂萃取和火焰原子化进行样品预浓缩的方法。在该计划的六个月期间，两个血样池中的每一个都获得了55个结果。使用了常用的统计方法和由尤登设计的图形系统进行数据分析。五个实验室的结果之间已获得可接受的一致性。已证实这类计划在提高分析熟练度方面的有用性。
