Spector I, Shochet N R, Kashman Y, Groweiss A
Science. 1983 Feb 4;219(4584):493-5. doi: 10.1126/science.6681676.
Two toxins, latrunculins A and B, which contain a new class of 16- and 14-membered marine macrolides attached to the rare 2-thiazolidinone moiety, were purified recently from the Red Sea sponge Latrunculia magnifica. The effects of these toxins on cultured mouse neuroblastoma and fibroblast cells have been evaluated. In both types of cells, submicromolar toxin concentrations rapidly induce striking changes in cell morphology that are reversible upon removal of the toxin. Immunofluorescence studies with antibodies specific for cytoskeletal proteins reveal that the toxins cause major alterations in the organization of microfilaments without obvious effects on the organization of the microtubular system.
最近从红海海绵巨大叶状海绵（Latrunculia magnifica）中纯化出了两种毒素，即拉春库林A和B，它们含有一类新型的与罕见的2-噻唑烷酮部分相连的16元和14元海洋大环内酯。已评估了这些毒素对培养的小鼠神经母细胞瘤和成纤维细胞的影响。在这两种类型的细胞中，亚微摩尔浓度的毒素会迅速引起细胞形态的显著变化，去除毒素后这些变化是可逆的。用针对细胞骨架蛋白的特异性抗体进行的免疫荧光研究表明，这些毒素会导致微丝组织发生重大改变，而对微管系统的组织没有明显影响。
