Boros D L, Lande M A
Am J Trop Med Hyg. 1983 Jan;32(1):78-82. doi: 10.4269/ajtmh.1983.32.78.
There is a dearth of knowledge on the tissue fibrosis that contributes to the pathology of schistosomiasis mansoni. The present study was designed to test the direct effect of live schistosome eggs and soluble egg antigens (SEA) on cultured normal human fibroblasts. Coincubation for 3 days of fibroblast monolayers with 100-500 live eggs/ml medium or equivalent amounts of SEA caused enhanced incorporation of labelled proline. The newly synthesized polypeptides were sensitive to purified collagenase enzyme activity. Collagen synthesis was also verified by measuring increased hydroxyproline content in fibroblasts. Whereas low numbers of eggs stimulated fibroblast activity, 500 eggs/ml medium caused a disarray in the arrangement of cells, with cytoplasmic granulation, cell detachment and death. These results indicate that fibroblast stimulation and collagen synthesis may also be triggered by the direct action of egg secretions on tissue fibroblasts.
关于导致曼氏血吸虫病病理变化的组织纤维化，目前缺乏相关知识。本研究旨在测试活血吸虫卵和可溶性虫卵抗原（SEA）对培养的正常人成纤维细胞的直接作用。将成纤维细胞单层与每毫升培养基中含100 - 500个活卵或等量SEA共同孵育3天，可导致标记脯氨酸的掺入增加。新合成的多肽对纯化的胶原酶活性敏感。通过测量成纤维细胞中羟脯氨酸含量的增加也证实了胶原蛋白的合成。虽然少量虫卵刺激成纤维细胞活性，但每毫升培养基中500个虫卵会导致细胞排列紊乱，出现细胞质颗粒化、细胞脱离和死亡。这些结果表明，虫卵分泌物对组织成纤维细胞的直接作用也可能引发成纤维细胞的刺激和胶原蛋白的合成。
