Owhashi M, Horii Y, Ishii A
Am J Trop Med Hyg. 1983 Mar;32(2):359-66. doi: 10.4269/ajtmh.1983.32.359.
Significant chemotactic activity for eosinophils was detected in soluble egg antigen (SEA) preparations of both Schistosoma japonicum and S. mansoni in dose-dependent fashion. The activity of S. japonicum SEA was higher than that of S. mansoni SEA. Gel filtration on Sephadex G-150 showed that S. japonicum SEA was composed of two groups of eosinophil chemotactic factors (ECFs), one of high molecular weight (JEE-H) and the other of low molecular weight (JEE-L). S. mansoni SEA showed ECF composition similar to that of S. japonicum SEA. JEE-H was stable on heating (100 degrees C, 60 min) and resistant to pronase digestion, but was sensitive to periodate oxidation. JEE-L was also stable on heating and resistant to pronase and carboxypeptidase A digestions. These properties of the ECFs were also held in common with those of S. mansoni SEA. JEE-L was extractable by toluene, indicating a hydrophobic nature. These results suggest that schistosome eggs themselves contain ECFs, and that the composition of S. mansoni and S. japonicum SEA-derived ECFs is essentially the same. However, they differ from the other ECFs which have already been described in schistosome infections.
在日本血吸虫和曼氏血吸虫的可溶性虫卵抗原（SEA）制剂中均检测到对嗜酸性粒细胞具有显著的趋化活性，且呈剂量依赖性。日本血吸虫SEA的活性高于曼氏血吸虫SEA。在Sephadex G - 150上进行凝胶过滤显示，日本血吸虫SEA由两组嗜酸性粒细胞趋化因子（ECF）组成，一组为高分子量（JEE - H），另一组为低分子量（JEE - L）。曼氏血吸虫SEA显示出与日本血吸虫SEA相似的ECF组成。JEE - H在加热（100℃，60分钟）时稳定，对链霉蛋白酶消化有抗性，但对高碘酸盐氧化敏感。JEE - L在加热时也稳定，对链霉蛋白酶和羧肽酶A消化有抗性。这些ECF的特性也与曼氏血吸虫SEA的特性相同。JEE - L可被甲苯提取，表明其具有疏水性。这些结果表明，血吸虫卵本身含有ECF，并且曼氏血吸虫和日本血吸虫SEA衍生的ECF组成基本相同。然而，它们与血吸虫感染中已描述的其他ECF不同。
